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KMID : 0379119930210030195
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Korean Journal of Mycology
1993 Volume.21 No. 3 p.195 ~ p.199
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Protoplast Fusion of Fusarium oxysporum and activity of polygalacturonase
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Abstract
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This experiments was designed for development of Fusarium asysporum strains with enhanced activity of polygalacturonase by means of mutants and protoplasts fusion. Six auxotrophic mutants of F. oxysporum were induced by treatment of MNNG. Protoplasts from mutants were formed from early exoponential mycetinm after treatment with driselase(10 ㎎/㎖ using 0.6 M KCl as osmotic stabilizer. Fusion experiments between protoplasts of several auxotrophic mutants were done using polyethyleneglycol 8,000 and CaCl₂. The frequency of fusion was 5.0×10^(-3) as determined from several experiments. Activity of polygalscturonase was determined by the methods of modified DNS. Consequently, the polygalacturonase activities of mutants and fusant derived F. oxysporum were 1.4 to 3.5 times greater than that of the parental strain, and mutant Fx-2 seemed to be the best strain. Thus, the method we used seemed to be favorable for the improvement of strains.
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